| Title |
Human oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification |
| Authors |
Steven F. Mullen, Mei Li, Yuan Li, Zi-Jiang Chen, and John K. Critser |
| Publication Info |
Techniques and Instrumentation 89/6/1812-1825 |
| Date Published |
Mar 3, 2007 |
| Abstract |
Objective: To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence
of ethylene glycol, and to use this information to develop a method to vitrify human oocytes.
Design: An incomplete randomized block design.
Setting: A university-affiliated assisted reproductive center.
Patient(s): Women undergoing assisted reproduction in the Center for Reproductive Medicine at Shandong
University.
Intervention(s): Oocytes were exposed to 1.0 molar ethylene glycol in a single step and photographed during subsequent
volume excursions.
Main Outcome Measure(s): A two-parameter model was employed to estimate the permeability to water and
ethylene glycol.
Result(s): Water permeability ranged from 0.15 to 1.17 mm/(min$atm), and ethylene glycol permeability ranged
from 1.5 to 30 mm/min between 7C at 36C. The activation energies for water and ethylene glycol permeability
were 14.42 Kcal/mol and 21.20 Kcal/mol, respectively.
Conclusion(s): Despite the lower permeability of human metaphase II oocytes to ethylene glycol compared with
previously published values for propylene glycol and dimethylsulfoxide, methods to add and remove human oocytes
with a vitrifiable concentration of ethylene glycol can be designed that prevent excessive osmotic stress and
minimize exposure to high concentrations of this compound. (Fertil Steril 2008;89:1812–25.2008 by American
Society for Reproductive Medicine.)
Key Words: Vitrification, ethylene glycol, human, oocytes, permeability, computer modeling |
| Article |
/pdfs/Human_oocyte_vitrification.pdf |
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