VEG Cryoprotectants is a low-toxicity cryoprotective formula designed for the successful vitrification of living systems. It is supplied as a mixture of pure cryoprotectants, and is used the same way as a pure cryoprotectant would be used to prepare vitrification solutions.
The intended use of VEG Cryoprotectant is to prepare the vitrification solution known as VEG, which contains 55% w/v (55 g/dl) of VEG mixture can be used at final working concentrations of about 52-57% w/v depending on the desired stability against ice formation. VEG can be combined with Super cool X-1000 and Super cool Z-1000 for additional stability against ice formation. It can also be used in combination with other additives to optimize their use for your particular system
Please note that this product does not contain a carrier solution. This allows you to prepare VEG -based vitrification solutions using your carrier solution of choice. If you prefer to purchase VEG prepared in a carrier solution and ready to use, please inquire.
VEG Cryoprotectant allows solutions and cells in them to be cooled to below the glass transition temperature without the formation of ice. VEG Cryoprotectant achieve an extraordinary lack of toxicity by allowing water to vitrify without making water unavailable to hydrate bimolecular in living cells. The VEG vitrification solution is the parent formula for a wide family of vitrification solutions that have been created to satisfy a broad range of needs in cryopreservation.
VEG Cryoprotectant at a concentration of 55-57% w/v in an LM5 carrier solution have been shown to allow mammalian tissue slices to be vitrified with 100% recovery of membrane ion transport function compared to untreated control tissue (unpublished results). A solution 95% as concentrated as full strength VEG (95% of 55% w/v VEG Cryoprotectant) plus 1% X-1000 has been shown to be more effective for the vitrification of osteoblasts than any competing solution, including 90% VS55.
For additional details or custom solutions to your cryopreservation problems, please contact us.
Fahy GM, Wowk B, Wu J, and Paynter S. 'Improved vitrification solutions based on the predictability of vitrification solution toxicity' Cryobiology. 2004 48:22-35
Liu, B.L., and McGrath, J. 'Vitrification solutions for the cryopreservation of tissue-engineered bone0.' Cell Preservation Technology (in press, 2004).
We would like to know how well VEG will work in your research application. Please contact us with questions or to let us know about your experience.