Super cool Z-1000 is an additive that suppresses ice formation to enhance the performance of vitrification solutions. It is a non-colligative “synthetic ice blocker” that specifically targets and inactivates bacterial ice nucleating proteins, even when present at very small concentrations. These proteins seem to be a ubiquitous environmental contaminant and generic ice blockers such as Super cool X-1000 are not completely effective against them. Z-1000 is best used in combination with X-1000 to “clean up” residual heterogeneous ice nucleation events in vitrification solutions.
Z-1000 is not a vitrification solution, although it can be used in concentrations of several percent as a non-penetrating component of vitrification solutions. It has not been tested for efficacy in freezing solutions, or in dilute vitrification solutions dominated by homogeneous ice nucleation.
Normally, ice crystallizes around tiny impurities called heterogeneous nucleators. In the past, extremely expensive antifreeze proteins were the only substances known to suppress this phenomenon. Super cool Z-1000 is a synthetic molecule that specifically binds the class of heterogeneous nucleators produced by ice-nucleating proteins.
We would like to know how well Super cool Z-1000 will work in your research application. Please contact us with questions or to let us know about your experience.